TABLE 2.
Genes of L. lactis IL1403 (NC 002662) and MG1363 (NC 009004) encoding enzymes with the highest degrees of identity to known HicDHs of lactobacilli and impact of their inactivation in TIL46 on HicDH activity
| IL1403 gene | MG1363 gene (% identity) | Homolog of gene product | Species containing homolog | % Identity of enzymes | HicDH activity in mutant strain (% of activity in wild-type strain)a |
|---|---|---|---|---|---|
| ldh | ldh (100) | l-HicDH | Lactobacillus confusus | 31 | 193 ± 70 |
| 1114110 (hicD) | Llmg_0475 (88) | l-HicDH | Lactobacillus confusus | 41 | 104 ± 16 |
| ldhB | ldhB (96) | l-HicDH | Lactobacillus confusus | 29 | 97 ± 17 |
| ldhX | ldhX (94) | l-HicDH | Lactobacillus confusus | 31 | 128 ± 20 |
| yugC | Llmg_2242 (97) | d-HicDH | Lactobacillus casei | 29 | 91 ± 14 |
a
Mutant strains were strains with the indicated gene inactivated. Data are means ± standard deviations.