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. 2008 Nov 21;191(3):890–897. doi: 10.1128/JB.01507-08

FIG. 3.

FIG. 3.

Gene promoter activity in X. oryzae pv. oryzae XKK.12 harboring the reporter plasmid and grown in different media with and without macerated rice. PYS, rich PYS medium; PYS-Rice-Medium, PYS rich medium with macerated rice; Rice-Medium, macerated rice in distilled sterile water; Infected-Rice-Medium, rice which was infected with X. oryzae pv. oryzae for 10 days prior to maceration (see text for details). (A) pip gene promoter activity in X. oryzae pv. oryzae XKK.12 harboring the reporter plasmid pPIP122. The highest pip promoter activity was observed when X. oryzae pv. oryzae XKK.12(pPIP122) was grown in the presence of infected rice. No promoter activity was detected in the oryR mutant X. oryzae pv. oryzae XKK.12ORY(pPIP122). The results are expressed as means ± standard deviations (n = 3). *, P < 0.002 compared to the parental strain; §, P < 0.003 compared to the parental strain; #, P < 0.003 compared to the parental strain; †, P < 4 × 10−5 compared to the parental strain. (B) oryR promoter activity in X. oryzae pv. oryzae XKK.12(pORY122) and in the oryR mutant X. oryzae pv. oryzae XKK.12ORY(pORY122). The results are expressed as means ± standard deviations (n = 3). *, P < 0.002 compared to the parental strain; §, P < 0.012 compared to the parental strain; #, P < 0.002 compared to the parental strain; †, P < 0.001 compared to the parental strain. (C) cbsA promoter activity in X. oryzae pv. oryzae XKK.12(pCBS122) in different growth media. No promoter activity was detected in the oryR mutant X. oryzae pv. oryzae XKK.12ORY(pCBS122). The results are expressed as means ± standard deviations (n = 3). *, P < 6 × 10−5 compared to the parental strain; §, P < 0.002 compared to the parental strain; #, P < 2 × 10−4 compared to the parental strain; †, P < 3 × 10−4 compared to the parental strain.