TABLE 1.
X. oryzae pv. oryzae strains, plasmids, and oligonucleotides used in this study
| Strain, plasmid, or oligonucleotide | Characteristics or sequence | Reference or source |
|---|---|---|
| Strains | ||
| X. oryzae pv. oryzae XKK.12 | Wild-type strain | 13 |
| X. oryzae pv. oryzae XKK.12ORYR | Strain XKK.12 OryR mutant | 13 |
| A. tumefaciens NTL4(pZLQR) | Indicator strain for AHL detection | 5 |
| Plasmids | ||
| pMOSBlue | Cloning vector, Ampr | Amersham-Pharmacia |
| pSS122 | Promoter probe vector, IncW, Apr Gmr | This study |
| pORY122 | oryR promoter cloned with HindIII-SmaI in pSS122 | This study |
| pPIP122 | pip promoter cloned with HindIII-PstI in pSS122 | This study |
| pCBS122 | cbsA promoter cloned with HindIII-SalI in pSS122 | This study |
| pRPFF122 | rpfF promoter cloned with PstI-SalI in pSS122 | This study |
| Oligonucleotides | ||
| UIDAS | 5′-CCGGTACCTTGACCAGTATTAT-3′ | This study |
| UIDAR | 5′-CAGAATTCTCATTGTTTGCCTC-3′ | This study |
| ORYPRS | 5′-ATAAGCTTAGACGCCGCCGAAG-3′ | This study |
| ORYPRR | 5′-ATCCCGGGTAGACCAACGACTG-3′ | This study |
| PIPPRS | 5′-TTAAGCTTCGCGTGATGCGCTTG-3′ | This study |
| PIPPRR | 5′-TTCTGCAGTGGCCGCCAGATCCT-3′ | This study |
| CBSPRS | 5′-TTAAGCTTGCGTGTGGGCGTCAG-3′ | This study |
| CBSPRR | 5′-TTGTCGACCGCGCCTGTCAGCAA-3′ | This study |
| RPFFPRS | 5′-AACTGCAGATCGCCACCATGC-3′ | This study |
| RPFFPRR | 5′-CAGTCGACCGTCGAATTCTAT-3′ | This study |