TABLE 3.
Distribution of LSPs across M. aviuma
| Species | Strain | Southern blot | Non map specific
|
M. avium subsp. paratuberculosis specific
|
M. avium subsp. paratuberculosis lineage specific
|
||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| LSPP 1 | LSPP 3 | LSPP 5 | LSPP 6 | LSPP 7e | LSPP 8f | LSPP 10 | LSPP 13 | LSPP 17 | LSPP 2b | LSPP 4 | LSPP 11 | LSPP 12 | LSPP 14 | LSPP 15 | LSPP 16c | LSPA 8 | GPLd | LSPA 4-II | MAV 14 | LSPA 18 | LSPA 20 | Deletion 2 | VA 15 | LSPA 11 | |||
| M. avium subsp. | |||||||||||||||||||||||||||
| hominissuis | 104 | Yes | - | − | − | i | − | − | − | − | − | − | − | − | i | − | − | − | + | 104 | + | + | + | + | + | + | + |
| MB063764 | Yes | + | − | − | + | − | − | + | − | − | − | − | − | i | − | − | − | + | + | + | + | + | + | + | + | + | |
| ATCC 49601 | Yes | − | − | + | i | + | − | + | + | + | − | i | − | i | − | − | − | + | 104 | + | + | + | + | + | + | + | |
| 76102 | Yes | − | − | + | i | + | + | + | i | + | − | − | − | i | − | − | − | + | 104 | + | + | + | + | + | + | + | |
| 108 | No | − | − | + | i | + | + | + | i | + | − | − | − | i | − | − | − | + | 104 | + | + | + | + | + | + | + | |
| DV | Yes | + | − | − | i | − | − | + | − | − | − | − | − | i | − | − | − | + | 104 | + | + | + | + | + | + | + | |
| 28132 | Yes | + | − | − | i | − | − | + | − | − | − | − | − | i | − | − | − | + | 104 | + | + | + | + | + | + | + | |
| MB064490 | Yes | + | − | − | i | − | − | + | − | − | − | − | − | i | − | − | − | + | 104 | + | + | + | + | + | + | + | |
| 2000-325 | No | − | + | + | + | + | − | + | i | − | − | − | ND | i | − | − | − | + | + | + | ND | + | + | + | + | + | |
| HMC36A | No | − | − | + | + | + | − | + | − | − | − | − | − | i | − | − | − | + | + | + | + | + | + | + | + | + | |
| avium | ATCC 25291T | Yes | − | − | + | + | − | + | + | i | − | − | i | − | i | − | − | − | + | + | + | i | + | + | + | + | + |
| ATCC 35713 | Yes | − | − | + | + | − | + | + | i | − | − | i | − | i | − | − | − | + | + | + | i | + | + | + | + | + | |
| ATCC 15796 | Yes | − | − | + | + | − | + | + | i | − | − | i | − | i | − | − | − | + | + | + | i | + | + | + | i | + | |
| silvaticum | ATCC 49884T | Yes | − | − | + | + | − | + | + | i | − | − | i | − | i | − | − | − | + | + | + | i | + | + | + | i | + |
| 9800851 | No | − | − | + | + | − | + | + | i | − | − | i | − | i | − | − | − | + | + | + | i | + | + | + | i | + | |
| paratuberculosis, cattle genotype | MAP K-10 | Yes | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | − | K-10 | − | − | − | + | + | + | + |
| 316F | Yes | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | − | K-10 | − | − | − | + | + | + | + | |
| MAP 119 | Yes | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | − | K-10 | − | − | − | + | + | + | + | |
| 7296 | Yes | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | − | K-10 | − | − | − | + | + | + | + | |
| paratuberculosis, sheep genotype | 6756 | Yes | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | − | + | + | + | + | − | − | + | + |
| 85/14 | Yes | + | + | + | + | + | + | + | + | + | + | + | + | + | +/- | + | + | − | + | + | + | + | − | − | i | + | |
| LN20 | Yes | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | − | + | + | + | + | − | − | + | − | |
| P465 | Yes | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | − | + | + | + | + | − | − | + | + | |
| M. intracellulareg | ATCC 13950T | No | − | − | + | − | − | − | + | MIh | − | − | − | − | − | − | − | MI | + | + | − | + | + | + | + | + | + |
+, Positive by multiplex PCR, LSP present; −, negative by multiplex PCR, LSP absent; i, internal target not detected, LSP absent; +/−, see the text (strain-specific deletion after acquisition of LSPP14). For the non-M. avium subsp. paratuberculosis-specific LSPs, the location previously described was absent in strain 104 (17). For the M. avium subsp. paratuberculosis-specific and M. avium subsp. paratuberculosis-lineage-specific LSPs, the locations can be found in Table S2 in the supplemental material. ND, not determined.
In negative strains, LSPP2 is replaced by the LSPA21 polymorphism.
In negative strains, LSPP16 is replaced by the LSPA24 polymorphism.
In negative strains, GPL conformation can match that found in M. avium subsp. paratuberculosis strain K-10 or M. avium subsp. hominissuis strain 104.
In negative strains, LSPP7 is replaced by the MAV15 polymorphism.
In negative strains, LSPP8 is replaced by the LSPA15 polymorphism, except for strain HMC36A, which exhibits a unique polymorphism.
In negative strains, some LSPs are replaced by M. intracellulare-specific polymorphisms.
MI, M. intracellulare.