TABLE 2.
Proposed functions and predicted orthologs for differentially expressed P. aerophilum genes
| Locus (annotation) | Proposed function | Conserved domaina | Predicted ortholog in Pyrobaculum species
|
|||
|---|---|---|---|---|---|---|
| P. arsenaticum | P. calidifontis | P. islandicum | T. neutrophilus | |||
| PAE1353 (alternative ORF) | O2-scavenging oxidoreductase | Region 5′ of Pcal_1950 | ||||
| PAE1355 (cytochrome c oxidase subunit II) | O2-scavenging oxidoreductase | cl08244: cytochrome c oxidase subunit II | Pcal_1950 | |||
| PAE1357 (cytochrome c oxidase subunit I) | O2-scavenging oxidoreductase | cd01660: ba3-like heme-copper oxidase subunit I | Pcal_1949 | |||
| PAE1336 (possible respiratory chain protein) | Aerobic O2 reductase | Pars_0585 | ||||
| PAE1337 (cytochrome c oxidase subunit I/III) | Aerobic O2 reductase | cl00275: heme-copper oxidase subunit I; cd00386: heme-copper oxidase subunit III | Pcal_1943 | |||
| PAE1338 (cytochrome c oxidase subunit II) | Aerobic O2 reductase | cl08244: cytochrome c oxidase subunit II | Pcal_1944 | |||
| PAE1340 (respiratory chain protein [SoxI homolog]) | Aerobic O2 reductase | Pcal_1945 | ||||
| PAE2679 (transcriptional regulator) | NO-responsive transcriptional regulator | cl00088: HTH ARSR superfamily | Pars_0504 | |||
| PAE2680 (hypothetical protein) | NO-responsive transcriptional regulator | COG3945: uncharacterized conserved protein | Pars_0505 | Pcal_1917 | ||
| PAE1888 (multicopper oxidase) | NO2− or N2O reductase | cl06664: multicopper oxidase | ||||
| PAE1263 (molybdopterin oxidoreductase, iron-sulfur-binding subunit) | Arsenate respiratory reductase | COG0437: HybA, Fe-S-cluster-containing hydrogenase | Pars_0387 | Pcal_1599 | Pisl_1986 | Tne_0510 |
| PAE1264 (hypothetical membrane protein) | Arsenate respiratory reductase | cl01295: polysulfide reductase integral transmembrane protein NrfD | Pars_0388 | Pcal_1600 | ||
| PAE1265 (molybdopterin oxidoreductase, molybdopterin-binding subunit) | Arsenate respiratory reductase | cd02758: MopB tetrathionate reductase; cd02780: molybdopterin-binding, C-terminal (MopB_CT) region of tetrathionate reductase (TtrA); respiratory arsenate As(V) reductase (ArrA) | Pars_0389 | Pcal_1601b | ||
| PAE2859 (molybdopterin oxidoreductase, molybdopterin-binding subunit) | Thiosulfate or polysulfide reductase | cd02755: MopB_thiosulfate-R-like; cd02775: molybdopterin-binding, C-terminal (MopB_CT) | Pars_0938 | Pcal_1500 | Pisl_0162 | Tne_1951 |
| PAE2860 (molybdopterin oxidoreductase, iron-sulfur subunit) | Thiosulfate or polysulfide reductase | COG0437: HybA, Fe-S-cluster-containing hydrogenase | Pars_0937 | Pcal_1499 | Pisl_0161 | Tne_1952 |
| PAE2861 (molybdopterin oxidoreductase, membrane subunit) | Thiosulfate or polysulfide reductase | cl01295: polysulfide reductase integral transmembrane protein NrfD | Pars_0936 | Pcal_1498 | ||
| PAE2696 (Mn catalase) | Iron-responsive regulon | cd01051: Mn catalase | Pars_1142 | Pcal_0729 | ||
| PAE2697 (putative ABC transport protein) | Iron-responsive regulon | cl00427: binding protein-dependent transport system inner membrane component | ||||
| PAE2699 [putative Fe(III) ABC transporter ATP-binding protein] | Iron-responsive regulon | cd03255: ABC_MJ0796_Lo1CDE_FtsE | ||||
| PAE2700 (putative ABC transport protein) | Iron-responsive regulon | cl02460: transferrin; cl00115: periplasmic binding protein (PBPb) | ||||
| PAE0600 (hypothetical protein) | Iron-responsive regulon | cl00437: ZIP zinc transporter | Pars_1201 | Pcal_1350 | ||
a
From the Conserved Domain Database (CDD). The CDD identifier and product are given. HTH, helix-turn-helix; ARSR, arsenical resistance operon repressor.
b
See Results and Discussion.