FIG. 1.

(A) Alignment of the amino acid sequence of gp65 (Uniprot entry VG65_BPMD2, O64257) of mycobacteriophage D29 with that of RadA/Sms (A4T5L4_MYCGI) of M. gilvum. The conserved motifs are indicated. The R residue that was mutated is shown in boldface. (B) SDS-12% PAGE analysis of recombinant gp65 (wild-type or mutant) fractions eluted from an Ni-NTA agarose column. In, So, and E1 to E3 indicate extracts of induced cells, the soluble supernatant, and three successive elutions (1 ml each) with buffer C, respectively. The position of the band corresponding to the recombinant gp65 (26 kDa) is indicated by an arrow in both cases.