FIG. 6.

Effect of the R203G mutation on gp65 nuclease activity. Structure 1 was either untreated (lane U) or treated with the indicated amounts of either wild-type gp65 or mutant protein as indicated on the top. B, Boiled substrate without gp65 treatment; B*, boiled substrate after treatment with a 2X amount of gp65. 1X, 0.5 μg of either gp65 or mutant. A solid arrow indicates the truncated substrate, and a broken arrow indicates the truncated labeled oligonucleotide released after boiling of the gp65-treated substrate. The arrow on the left points to the unannealed oligonucleotide band.