FIG. 7.

Expression of gene 65 after the induction of M. smegmatis L1c1ts lysogen. A culture of M. smegmatis L1c1ts grown at 22°C was immersed in a 42°C water bath for thermoinduction. Aliquots were removed at designated intervals. The cells pellets were processed for immunoblot analysis (A). Lane C represents 1 μg of purified protein. The arrow indicates the band corresponding to gp65 (26 kDa). The phage titer in the corresponding supernatants was determined, and the number of PFU present per ml of supernatant was plotted against time. The resulting one-step growth curve is shown in panel B.