TABLE 5.

Effects of control region, carbon source, cAMP, and nitrate on Φ(napF-lacZ) expression

Control region alteration		NO3−b	LacZ sp act (Miller units)c				Sp act ratiod
P1	P2		Mannose	Glucose	+cAMP	-cAMP	Carbon	cAMP
+	+	-	1,090	280	430	170	3.9	2.5
+	-	-	320	210	540	120	1.5	4.5
-	+	-	1,260	240	740	200	5.2	3.7
+	+	+	16,980	9,360	7,970	4,360	1.8	1.8
+	-	+	21,550	15,350	12,090	9,390	1.4	1.3
-	+	+	490	100	120	70	4.9	1.7

^a Control region alteration in Φ(napF-lacZ) construct (Fig. 1). The genotype for strains to compare mannose versus glucose is narQ⁺ narP⁺ narX⁺ narL215::Tn10 λΦ(napF-lacZ) [Δ146]. The genotype for strains to determine the effect of cAMP is narQ⁺ narP⁺ narX⁺ narL215::Tn10 λΦ(napF-lacZ) [Δ146] Δcya-854 (Table 1).

^bPresence (+) or absence (-) of 40 mM nitrate during growth.

^cStrains were cultured anaerobically to the early exponential phase in MOPS defined medium. The carbon source was added (40 mM) as indicated, and cAMP was added (5 mM) as indicated. Cultures to test cAMP effects contained glucose as the sole carbon source.

^dRatio of LacZ specific activities between cultures with mannose and glucose or with and without added cAMP.