TABLE 6.

Effects of control region, carbon source, and Crp on Φ(napF-lacZ) expression in the absence of nitrate

Straina	Control region alterationb		crp allelec	LacZ sp act (Miller units)d		Catabolite repression (fold)	Crp activation (fold)
	P1	P2		Fructose	Glucose		Fructose	Glucose
VJS5516	+	+	+	1,120	600	1.9	7.0	3.3
VJS11445	+	+	-	160	180	0.9
VJS7444	+	-	+	680	560	1.2	4.0	2.2
VJS11447	+	-	-	170	250	0.7
VJS7443	-	+	+	540	150	3.6	6.0	1.9
VJS11446	-	+	-	90	80	1.1

^aGenotype: narQ⁺ narP⁺ narX⁺ narL215::Tn10 λΦ(napF-lacZ) [Δ146] (Table 1).

^bControl region alteration in Φ(napF-lacZ) construct (Fig. 1).

^c+, crp⁺; -, Δcrp-45.

^dStrains were cultured anaerobically to the early exponential phase in MOPS defined medium supplemented with 0.2% acid-hydrolyzed casein. The carbon source was added (40 mM) as indicated.