TABLE 3.
RpoS- and DsrA-dependent gene mRNA levels at 37 and 23°C in wild-type and hns651 mutant strainsa
| Gene(s) | Wild type
|
hns651 mutant
|
23/37°C ratio
|
hns651/wild-type ratio
|
||||
|---|---|---|---|---|---|---|---|---|
| 37°C | 23°C | 37°C | 23°C | Wild type | hns651 mutant | 37°C | 23°C | |
| rpoS | 1.0 (0.9-1.1) | 1.9 (1.5-2.4) | 0.7 (0.5-1.0) | 2.5 (1.8-3.4) | 1.9 | 3.6 | 0.7 | 1.3 |
| dsrA | 1.0 (0.7-1.4) | 5.6 (3.5-8.8) | 4.6 (2.9-7.2) | 11.7 (8.7-15.8) | 5.6 | 2.5 | 4.6 | 2.1 |
| bolA | 1.0 (0.8-1.2) | 5.5 (3.8-7.9) | 2.9 (2.2-3.9) | 12.8 (10.8-15.3) | 5.5 | 4.4 | 2.9 | 2.3 |
| csgA | 1.0 (0.7-1.4) | 745.7 (610.8-910.4) | 10.9 (6.5-18.0) | 135.8 (97.1-190) | 746 | 12.5 | 10.9 | 0.2 |
| nhaR | 1.0 (0.9-1.2) | 3.9 (2.9-5.2) | 2.5 (1.7-3.8) | 2.8 (2.0-4.0) | 3.9 | 1.1 | 2.5 | 0.7 |
| otsA | 1.0 (0.8-1.3) | 3.0 (2.4-3.9) | 11.6 (9.4-14.3) | 13.2 (9.3-18.8) | 3.0 | 1.1 | 11.6 | 4.4 |
| yceP/bssS | 1.0 (0.9-1.1) | 3.6 (2.7-4.7) | 3.2 (2.3-4.4) | 13.6 (4.8-39.2) | 3.6 | 4.3 | 3.2 | 3.8 |
| ycgZ | 1.0 (0.9-1.1) | 21.1 (17.8-24.9) | 5.1 (3.7-7.0) | 16.1 (13.6-19.0) | 21.1 | 3.2 | 5.1 | 0.8 |
| yhiM | 1.0 (0.6-1.7) | 7.1 (4.3-11.8) | 38.3 (22.9-63.8) | 225.0 (149.4-338.8) | 7.1 | 5.9 | 38.3 | 31.7 |
| ymdA | 1.0 (0.6-1.7) | 30.5 (15.1-61.9) | 1.8 (1.0-3.2) | 6.1 (4.1-9.1) | 30.5 | 3.4 | 1.8 | 0.2 |
a
Gene expression levels were measured by qRT-PCR. For each gene, the average expression level is in bold and is relative to the level measured at 37°C in wild-type strain DL1504. Values in parentheses are standard deviations based on the results of three independent experiments.