TABLE 2.

Transcriptional changes in isoprenoid-utilizing pathways

Function and RN4220 ORF (SAOUHSC no.)	Gene	Description	Fold changea
			S-/S+	A-/A+	K-/K+	WT-/WT+
IPP synthesis
02860	mvaS	HMG-CoA synthase	0.11	1.01	1.18	0.88
02859	mvaA	HMG-CoA reductase	1.41	0.15	1.11	0.90
00577	mvaK1	Mevalonate kinase	1.67	2.40	0.39	0.96
00578	mvaD	Diphosphomevalonate decarboxylase	1.69	1.96	0.08	0.83
00579	mvaK2	Phosphomevalonate kinase	1.48	1.62	0.11	0.79
UPP synthesis
02623	fni	Isopentenyl diphosphate isomerase	0.76	0.72	0.62	0.85
01618	ispA	Farnesyl pyrophosphate synthase	1.27	1.25	0.86	0.95
01237	upps	Undecaprenyl diphosphate synthase	1.86	2.26	1.85	0.97
00691	bacA	Undecaprenyl pyrophosphate phosphatase	1.86	2.70	2.01	0.82
Menaquinone synthesis
00982	menF	Isochorismate synthase	0.91	0.75	0.76	0.90
00983	menD	2-Succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase	0.94	0.60	0.60	0.87
01915	menC	O-Succinylbenzoic acid synthase	0.93	0.82	0.69	1.12
01916	menE	O-Succinylbenzoic acid-CoA ligase	1.42	1.34	1.19	1.02
00985	menB	Napthoate synthase	0.91	0.76	0.80	0.98
00984		Alpha/beta hydrolase	0.90	0.63	0.55	0.90
00980	menA	1,4-Dihydroxy-2-napthodate octaprenyltransferase	1.10	1.00	0.80	0.80
01487	menG	Methyltransferase	1.09	1.23	0.96	1.00
01486	hepB	Heptaprenyl diphosphate syntase	1.10	1.19	1.10	0.93
Heme A synthesis
01066	ctaB	Protoheme IX farnesyltransferase	0.78	0.77	0.70	0.98
01065	ctaA	Heme A synthase	0.41	0.47	0.42	0.91
Prenylated tRNA synthesis
01280	miaA	tRNA Δ2-isopentenylpyrophosphate transferase	1.33	1.82	1.32	0.97
Staphyloxanthin synthesis
02879	crtM	Squalene synthase	0.65	0.58	0.46	0.96
02877	crtN	Squalene desaturase	0.78	0.68	0.54	0.99
02880	crtQ	Glycosyltransferase	0.46	0.37	0.32	0.97
02881	crtP	Phytoene dehydrogenase	0.47	0.38	0.36	0.97
02882	crtO	Acyltransferase	0.61	0.44	0.41	1.23

^aS refers to RN4220spacmvaS, A refers to RN4220spacmvaA, K refers to RN4220spacK, and WT refers to RN4220. “+” refers to cells grown in the presence of 1 mM IPTG; “-” refers to cells grown in the absence of IPTG. The greatest changes in isoprenoid-utilizing pathways occur in the mvaK operon, upps, bacA, and the crt operon.