TABLE 3.
qRT-PCR quantification of mevalonate pathway transcriptional changes
| RN4220 ORF (SAOUHSC no.) | Gene | Fold changea
|
|||
|---|---|---|---|---|---|
| S-/S+ | A-/A+ | K-/K+ | WT-/WT+ | ||
| 02860 | mvaS | 0.18† | 1.46† | 1.11§ | 0.97§ |
| 02859 | mvaA | 1.1§ | 0.29† | 1.03† | 0.98† |
| 00577 | mvaK1 | 1.44† | 2.18† | 0.62† | 1.01† |
| 00578 | mvaD | 1.69† | 1.87† | 0.23† | 0.95† |
| 00579 | mvaK2 | 1.53† | 1.85† | 0.25† | 0.91† |
| 02243 | 4.63† | 15.24† | 5.62† | 1.1§ | |
| 02681 | narG | 0.08† | 0.19† | 0.13† | 1.12§ |
| 16S rRNA | 1.00 | 1.00 | 1.00 | 1.00 | |
S refers to RN4220spacmvaS, A refers to RN4220spacmvaA, K refers to RN4220spacK, and WT refers to RN4220. “+” refers to cells grown in the presence of 1 mM IPTG; “-” refers to cells grown in the absence of IPTG.
P < 0.005
P < 0.05.