TABLE 5.

Transcriptional changes in l-glutamate- and l-lactate-utilizing genes

Enzyme type and RN4220 ORF (SAOUHSC no.)	Gene	Description	Fold changea
			S-/S+	A-/A+	K-/K+	WT-/WT+
Glutamate-utilizing enzymes
00375	guaA	GMP synthase	0.66	0.43	0.42	0.89
01287	femC	Glutamine synthetase	0.52	0.39	0.45	0.97
00509	gltX	Glutamyl-tRNA synthetase	0.66	0.55	0.48	0.91
01014	purF	Amidophosphoribosyltransferase	0.26	0.14	0.14	1.01
00895	gluD	Glutamate dehydrogenase	0.78	0.41	0.62	0.97
01169	carA	Carbamoyl-phosphate synthase	0.10	0.15	0.10	1.10
01129	arcC1	Carbamate kinase	0.24	0.18	0.18	1.19
00008	hutH	Histidine ammonia-lyase	0.89	0.37	0.47	0.93
02606	hutI	Imidazolonepropionase	0.67	0.16	0.38	1.06
02607	hutU	Urocanate hydratase	0.60	0.21	0.41	1.08
02610	hutG	Formiminoglutamase	0.61	0.59	0.56	1.09
01776	hemA	Glutamyl-tRNA reductase	0.57	0.58	0.59	1.00
Lactate-utilizing enzyme
00206	ldh-1	l-Lactate dehydrogenase	0.38	0.65	0.43	1.07

^aS refers to RN4220spacmvaS, A refers to RN4220spacmvaA, K refers to RN4220spacK, and WT refers to RN4220. “+” refers to cells grown in the presence of 1 mM IPTG; “-” refers to cells grown in the absence of IPTG.