TABLE 6.
Transcriptional changes in glycolysis
| RN4220 ORF (SAOUHSC no.) | Gene | Description | Fold changea
|
|||
|---|---|---|---|---|---|---|
| S-/S+ | A-/A+ | K-/K+ | WT-/WT+ | |||
| 01646 | glcK | Glucokinase | 0.73 | 0.55 | 0.54 | 0.95 |
| 00900 | pgi | Glucose-6-phosphate isomerase | 1.62 | 1.32 | 1.54 | 0.91 |
| 01807 | pfk | 6-Phosphofructokinase | 1.13 | 1.45 | 1.12 | 0.96 |
| 02366 | fbaA | Fructose-bisphosphate aldolase | 0.78 | 0.76 | 0.69 | 1.00 |
| 00797 | tpi | Triosephosphate isomerase | 0.69 | 1.74 | 0.59 | 0.99 |
| 00795 | gap | Glyceraldehyde-3-phosphate dehydrogenase | 1.32 | 1.98 | 1.13 | 0.90 |
| 00796 | pgk | Phosphoglycerate kinase | 0.87 | 2.13 | 0.72 | 1.01 |
| 00798 | pgm | 2,3-Diphosphoglycerate-independent phosphoglycerate mutase | 0.54 | 1.81 | 0.50 | 0.98 |
| 00799 | eno | Enolase | 0.92 | 1.15 | 0.88 | 1.06 |
| 01806 | pykA | Pyruvate kinase | 0.97 | 1.01 | 0.94 | 0.99 |
| 01041 | pdhB | Pyruvate dehydrogenase E1 | 0.79 | 0.56 | 0.58 | 1.01 |
| 01043 | pdhD | Dihydrolipoamide dehydrogenase E3 | 0.72 | 0.49 | 0.58 | 0.98 |
| 01042 | pdhC | Dihydrolipoamide S-acetyltransferase E2 | 0.79 | 0.58 | 0.65 | 1.02 |
a
S refers to RN4220spacmvaS, A refers to RN4220spacmvaA, K refers to RN4220spacK, and WT refers to RN4220. “+” refers to cells grown in the presence of 1 mM IPTG; “-” refers to cells grown in the absence of IPTG.