TABLE 7.
Transcriptional changes in autolysis
| RN4220 ORF (SAOUHSC no.) | Gene | Description | Fold changea
|
|||
|---|---|---|---|---|---|---|
| S-/S+ | A-/A+ | K-/K+ | WT-/WT+ | |||
| 00994 | atl | Bifunctional autolysin | 0.59 | 0.42 | 0.31 | 0.87 |
| 00427 | N-Acetylmuramoyl-l-alanine amidase | 0.47 | 0.66 | 0.31 | 0.86 | |
| 00671 | Secretory antigen SsaA homologue | 0.75 | 0.85 | 0.66 | 1.01 | |
| 01219 | lytN | Cell wall hydrolase | 0.96 | 0.65 | 0.62 | 1.04 |
| 00998 | fmt | Autolysis and methicillin resistant-related protein | 2.50 | 3.77 | 2.38 | 0.93 |
a
S refers to RN4220spacmvaS, A refers to RN4220spacmvaA, K refers to RN4220spacK, and WT refers to RN4220. “+” refers to cells grown in the presence of 1 mM IPTG; “-” refers to cells grown in the absence of IPTG.