FIG. 7.
Proteinase K susceptibility of IcsA on intact and chloroform-treated cells. Western blots are for cell pellets treated with proteinase K (ProtK), either with or without prior disruption the outer membrane with chloroform (CHCl3). (A) Blot probed with antiserum to IcsA. (B) Blot probed with antiserum to DsbA. IcsA53-1102, full-length mature IcsA; IcsA53-757, proteolytically processed mature amino terminus of IcsA. Molecular size markers are indicated in kilodaltons to the left of the blot.