FIG. 8.
Heat modifiability of the IcsA β-barrel in the skp mutant and other strains. Western blot results are for IcsA in whole-cell protein preparations run on an SDS-PAGE gel after heating to 100°C or 30°C, as indicated above the blot. The blot was probed with antiserum to IcsA. uIcsA53-1102, unfolded full-length mature IcsA; fIcsA53-1102, folded full-length mature IcsA; IcsA53-757, proteolytically processed mature amino terminus of IcsA. Molecular size markers are indicated in kilodaltons to the left of the blot.