TABLE 3.
Transformation frequencies in various mutants
| Expt | Strain | Relevant genotype | No. of viable cells/ml (106)a | No. of transformed cells/ml (10)a | Frequency (10−4) | Relative frequency (%) |
|---|---|---|---|---|---|---|
| Screening test (second round) | 168 | Wild type | 314 | 2,400 | 0.76 | 100 |
| YRZDc | comN::Cmr | 114 | 0 | 0 | 0 | |
| YUTBd | yutB::Emr | 172 | 14 | 0.008 | 1.1 | |
| Backcross expt and transformation test using newly constructed strain | ||||||
| Expt 1 | 168 | Wild type | 206 | 15,600 | 7.6 | 100 |
| YUTBd | yutB::Emr | 124 | 840 | 0.68 | 9 | |
| Expt 2 | 168 | Wild type | 374 | 35,100 | 9.4 | 100 |
| OAM304 | yutB::Pmr | 203 | 2,930 | 1.4 | 15 | |
| Expt 3 | 168 | Wild type | 232 | 21,200 | 9.1 | 100 |
| YRZDc | comN::Cmr | 112 | 57 | 0.051 | 0.56 | |
| Complementation test | 168 | Wild type | 193 | 19,300 | 10 | 100 |
| YRZDc | comN::Cmr | 162 | 72 | 0.043 | 0.43 | |
| OAM434 | comN::CmramyE::comN | 248 | 15,600 | 6.3 | 63 | |
| OAM304 | yutB::Pmr | 138 | 1,600 | 1.2 | 12 | |
| OAM435 | yutB::PmramyE::yutB | 178 | 19,800 | 11 | 110 |
a
The numbers of colonies on LB agar plates containing serially diluted cultures were counted, and the results were calculated to the third significant digit.