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Size of the truncated version of Glk. Proteins were analyzed by SDS-PAGE, followed by Western blotting with anti-Glk antibodies. Lane 1, wild-type MG1655; lane 2, JW2385 (Δglk); lane 3, JW2385 harboring glk15 (Δ305-321); lane 4, JW2385 harboring plasmid-encoded N-terminal His6-tagged wild-type Glk under IPTG induction.
