TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype or descriptiona | Source or reference |
|---|---|---|
| E. coli strains | ||
| AS40 | MC4100 ΔmalB107 trp::[Kanr-malEΔp92-lacZ]opmalT+opmR::Tn10 | 50 |
| AS54 | MC4100 ΔmalB107 trp::[Kanr-malEΔp92-lacZ]opmalT(T38R) opmR::Tn10 | 50 |
| AS53 | MC4100 ΔmalB107 trp::[Kanr-malEΔp92-lacZ]opmalT(D65E) opmR::Tn10 | 50 |
| BRE1162 | MC4100 φ(malK-lacZ)1113(λplacMu50) | 5 |
| CL19 | Bre1162 ΔglgA::frtglk15::Tn10 (Cam) mlc::tet | This study |
| CL27 | Bre1162 glgA::Tn10 ΔmalZ::frt Δglk::kan | This study |
| CL28 | Bre1162 ΔmalZ::frt Δglk::kan | This study |
| GW11 | Bre1162 ΔmalZ::frtglgA::Tn10 Δ(malQ malP)::frt glk15::Tn10 (Cam) | This study |
| GW12 | Bre1162 ΔmalZ::frtglgA::Tn10 Δ(malQ malP)::frt glk15::Tn10 (Cam) mlc::kan | This study |
| GW15 | Bre1162 ΔmalZ::frtglgA::Tn10 ΔmalP::kan glk15::Tn10 (Cam) | This study |
| GW26 | MC4100 ΔmalB107 trp::[Kanr-malEΔp92-lacZ]opmalT+opmR::Tn10 ΔglgA::cam | This study |
| GW27 | MC4100 ΔmalB107 trp::[Kanr-malEΔp92-lacZ]opmalT(T38R) opmR::Tn10 ΔglgA::cam | This study |
| GW28 | MC4100 ΔmalB107 trp::[Kanr-malEΔp92-lacZ]opmalT(D65E) opmR::Tn10 ΔglgA::cam | This study |
| JM101 | Δ(lac-proAB) thi-1supE F′[traD36proAB+lacIqlacZΔM15] | 37 |
| JM-G77 | JM101 ΔptsG::cat ΔptsHI crr::kan ptsG-lacZ in the λ attachment site | 37 |
| JW2385 | Δglk::kan, derivative of MG1655 | NBRP (NIG, Japan): E. coli |
| M15 | F−nalS strS rifS thi lac mtl | Qiagen |
| MAD103 | Bre1162 ΔmalZ::frt glgA::Tn10 | This study |
| MAD104 | Bre1162 ΔmalZ::frtglk15::Tn10 (Cam) | This study |
| MAD106 | Bre1162 ΔmalZ::frtglgA::Tn10 glk15::Tn10 (Cam) | This study |
| MAD107 | Bre1162 ΔmalZ::frtglk15::Tn10 (Cam) ΔmalQ::frt | This study |
| MAD108 | Bre1162 ΔmalZ::frtglgA::Tn10 glk15::Tn10 (Cam) ΔmalQ::kan | This study |
| MC4100 | araD139 deoC1 flbB5301 ptsF25 rbsR relA1 rpsL150 Δ(argF-lac)U169 | 8 |
| MG1655 | Laboratory wild-type strain | |
| RD38 | Bre1162 ΔmalZ::kanglgA::Tn10 | 15 |
| RD103 | Bre1162 ΔmalZ::kan ΔglgA::frt malP::Tn10 | This study |
| RD105 | Bre1162 ΔmalZ::kan ΔglgA::frt glk15::Tn10 (Cam) malP::Tn10 | This study |
| RD126 | Bre1162 ΔmalZ::frtglgA::Tn10 Δ(malQ malP)::kan | This study |
| RD129 | Bre1162 ΔmalZ::frt Δ(malQ malP)::kan | This study |
| TB04 | Bre1162 ΔmalZ::kan | 15 |
| TB48 | Bre1162 ΔglgA::cammalT::Tn5 | This study |
| Plasmids | ||
| pREP4 | pACYC derivative containing the p15A replicon and lacIq; Kanr | Qiagen |
| pMAD145 | pQE70 (Qiagen) C-terminal His6-tagged MalQ | This study |
| pBK1 | Derivative of pGDR11 lacIq N-terminal His6 tag Glk; Ampr | Laboratory collection |
| pGDR11 | pQE31 (Qiagen) lacIq N-terminal His6 tag vector; Ampr | 36 |
| pTSG11 | Expresses ptsG under lacIq and under its own promoter | 6 |
| pCSF2 | Derivative of pACYC, glk+ under natural promoter; Kanr | 27 |
| pCSF34 | Derivative of pACYC, glk15 (Δ305-321) under natural promoter; Kanr | 27 |
| pIA1 | TTC1688 (encoding glucokinase of T. thermophilus) cloned into pGDR11; Ampr | This study |
a
Ampr, ampicillin resistance; Kanr, kanamycin resistance.