TABLE 6.
Differentiating the function of Glk in controlling mal gene expression
| Straina | Additional genotype | MalK-LacZ activity |
|---|---|---|
| MAD104 (glgA+) | glk15::Tn10 (Cam) vector | 4.21 |
| MAD104 (glgA+) | glk15::Tn10 (Cam) pglk+ | 0.08 |
| MAD104 (glgA+) | glk15::Tn10 (Cam) pTtglk+ | 1.59 |
| MAD106 | glk15::Tn10 (Cam) | 2.16 |
| MAD106 | glk15::Tn10 (Cam) pglk+ | 0.09 |
| MAD106 | glk15::Tn10 (Cam) pCSF34 (glk15 Δ306-321) | 0.88 |
| MAD106 | glk15::Tn10 (Cam) pCSF2glk+ | 0.58 |
| MAD106 | glk15::Tn10 (Cam) pTtglk+ | 1.20 |
| RD105 | glk15::Tn10 (Cam) malP | 1.95 |
| RD105 | glk15::Tn10 (Cam) malP vector | 2.02 |
| RD105 | glk15::Tn10 (Cam) malP pglk+ | 0.09 |
| RD105 | glk15::Tn10 (Cam) malP pTtglk+ | 1.05 |
| GW12 | glk15::Tn10 (Cam) malP malQ mlc vector | 4.27 |
| GW12 | glk15::Tn10 (Cam) malP malQ mlcpglk+ | 3.94 |
| GW12 | glk15::Tn10 (Cam) malP malQ mlcpTtglk+ | 4.39 |
| CL19 (malZ+) | glk15::Tn10 (Cam) mlc vector | 6.30 |
| CL19 (malZ+) | glk15::Tn10 (Cam) mlc pglk+ | 0.71 |
| CL19 (malZ+) | glk15::Tn10 (Cam) mlc pCFS2 (glk+) | 3.90 |
| CL19 (malZ+) | glk15::Tn10 (Cam) mlc pCSF34 (glk15 Δ306-321) | 4.75 |
| CL19 (malZ+) | glk15::Tn10 (Cam) mlc pTtglk+ | 6.37 |
a
All strains harbor malK-lacZ and lack glgA and malZ unless specified otherwise.