TABLE 2.
Intracellular cAMP contents in various V. vulnificus strains grown in LBS medium
| Growth phase | cAMP concn (pmol/mg protein) (SD)a
|
||||
|---|---|---|---|---|---|
| Wild type | Δcya | ΔcpdA | ΔcpdA (pLAFR5) | ΔcpdA (pHS51)b | |
| Exponentialc | 26.2 (3.6) | ND | 98.8 (7.1) | 137.3 (16.7) | ND |
| Stationaryd | 6.3 (0.8) | ND | 36.5 (0.2) | 44.7 (0.7) | ND |
a
Intracellular cAMP concentration (moles of cAMP in the unit mass of bacterial cells determined by the protein amount in lysate) was expressed as an average value from four independent experiments, with the standard deviation in parentheses. ND, the cAMP concentration was not detectable because it was below the detection limit of the assay used in this study.
b
A broad-host-range vector, pLAFR5-based plasmid DNA including the V. vulnificus cpdA gene.
c
V. vulnificus cells freshly grown in LBS medium at an OD595 ranging from 0.6 to ∼0.7.
d
V. vulnificus cells grown in LBS medium at an OD595 ranging from 3 to ∼4.