TABLE 2.
Distances between selected residues in MalF and MalE as determined by cross-linking and Cβ-Cβ distances from the X-ray structure of the maltose ABC transporter
| Residues in MalF/MalE | Distance (Å) between residues in:
|
||||
|---|---|---|---|---|---|
| MalFGK(E159Q)2-E (Cβ-Cβ)a | MalF500 | Wild type in indicated state
|
|||
| ATP-bound | Apo or posthydrolysis | Vanadate-trapped | |||
| S205C/T80C | 8.6 | NDb | ∼5 | ∼5 | ∼5 |
| T177C/T31C | 5 | >5-10 | ∼5 | >5-10 | >5-10 |
| S205C/T31C | 21 | >10-25 | >25 | >25 | >10-25 |
a
Determined from the X-ray structure (Protein Data Bank number 2R6G) with DS ViewerPro 6.0 (Accelrys, Cambridge, United Kingdom).
b
ND, not determined.