TABLE 1.

Oligonucleotide primers used to amplify the genes targeted for insertional mutagenesis

Primer	Sequencea
nocR-For	TATGAATTCTGGAGATGCGCGCGGGCGGG
nocR-Rev	TATGAATTCTCACGGGCCGGTCGTGGCAAGTT
nocD-For	GAATTCGTGACGCTGGTCAGCGGCA
nocD-Rev	GAATTCTCACCCGCCGGGCTCGC
nocE-For	AAGAATTCATGCGGTTTGTCAGAGCGATCGCG
nocE-Rev	AAGAATTCTCACTGTCCCCAGCTGTGCGACG
nocO-For	CTCGAGGCTGCGCGATCCCGAACCG
nocO-Rev	GAATTCGCCCGAGGCTGCGCGATCC

^aUnderlined portions of sequences indicate restriction enzyme sites.