TABLE 5.
Oligonucleotide primers for amplification of subsections of the nocF-nocA intergenic region
| Label | Primer sequence |
|---|---|
| Full region forward | TCACGAACCTCCAGCCGTGG |
| Full region reverse | AGTCCGCTCAGGCTAGGAAAA |
| 10 to 126 forward | TCCAGCCGTGGGATCGCCCTC |
| 20 to 126 forward | GGATCGCCCTCCGGC |
| 30 to 126 forward | CCGGCAGCATTTATAGACGGT |
| 40 to 126 forward | TTATAGACGGTTTGCTTATCGATCCGT |
| 50 to 126 forward | TTTGCTTATCGATCCGTTTTCAC |
| 60 to 126 forward | GATCCGTTTTCACATTCACCCG |
| AF 58-18 For | TCGATCCGTTTTCACATTCACCCGCAGTGATAAGGAATTGATAAACGATTTTCCTAGCCTG |
| AF 58-118 Rev | CAGGCTAGGAAAATCGTTTATCAATTCCTTATCACTGCGGGTGAATGTGAAAACGGAT |
| AF 48-108 For | GGTTTGCTTATCGATCCGTTTTCACATTCACCCGCAGTGATAAGGAATTGATAAACGATTT |
| AF 48-108 Rev | AAATCGTTTATCAATTCCTTATCACTGCGGGTGAATGTGAAAACGGATCGATAAGCAAA |
| AF 38-98 For | ATTTATAGACGGTTTGCTTATCGATCCGTTTTCACATTCACCCGCAGTGATAAGGAA |
| AF 38-98 Rev | CAATTCCTTATCACTGCGGGTGAATGTGAAAACGGATCGATAAGCAAACCGTCTATAAAT |
| AF 28-88 For | CTCCGGCAGCATTTATAGACGGTTTGCTTATCGATCCGTTTTCACATTCACCCGCAG |
| AF 28-88 Rev | TCACTGCGGGTGAATGTGAAAACGGATCGATAAGCAAACCGTCTATAAATGCTGCCGGAG |
| AF 18-78 For | TCGATCCGTTTTCACATTCACCCGCAGTGATAAGGAATTGATAAACGATTTTCCTAGCCTG |
| AF 18-78 Rev | CAGGCTAGGAAAATCGTTTATCAATTCCTTATCACTGCGGGTGAATGTGAAAACGGAT |
| AF 8-68 For | GGTTTGCTTATCGATCCGTTTTCACATTCACCCGCAGTGATAAGGAATTGATAAACGATTT |
| AF 8-68 Rev | AAATCGTTTATCAATTCCTTATCACTGCGGGTGAATGTGAAAACGGATCGATAAGCAAA |