TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype | Reference or source |
|---|---|---|
| E. chrysanthemi | ||
| A350 | Lac− derivative from 3937 | 25 |
| A350 outF | A350 ΔoutF | G. Condemine, laboratory collection |
| A350 outD | A350 ΔoutD | G. Condemine, laboratory collection |
| A350 outE | A350 ΔoutE | G. Condemine, laboratory collection |
| A350 outL | A350 ΔoutL | G. Condemine, laboratory collection |
| A350 outJ | A350 outJ::aphA3 (Kanr) | This study |
| A1077 | A350 kdgR::MudIPR13 (Cmr) | N. Hugouvieux, laboratory collection |
| V1077 | A350 ΔoutFkdgR::MudIPR13 (Cmr) | This study |
| 3937 cbsE | 3937 cbsE-1 (Spcr) | 33 |
| A350 cbsE | A350 cbsE-1 | This study |
| A350 cbsEoutF | A350 cbsE-1 ΔoutF | This study |
| A350 cbsEoutD | A350 cbsE-1 ΔoutD | This study |
| A350 cbsEoutE | A350 cbsE-1 ΔoutE | This study |
| A350 cbsEoutL | A350 cbsE-1 ΔoutL | This study |
| A350 cbsEoutJ | A350 cbsE-1outJ::aphA3 | This study |
| 3937 acsD | 3937 acsD-uidA (Kanr) | 16 |
| 3937 fur | 3937 fur::Ω | 18 |
| A350 acsD | A350 acsD-uidA | This study |
| A350 acsDoutF | A350 acsD-uidA ΔoutF | This study |
| A350 acsDfur | A350 acsD-uidAfur::Ω | This study |
| A350 acsDfur outF | A350 acsD-uidAfur::Ω ΔoutF | This study |
| 3937 fct | 3937 fct-lacZ34 (Kanr) | 33 |
| A350 fct | A350 fct-lacZ34 (Kanr) | This study |
| A350 fctoutF | A350 fct-lacZ34 ΔoutF | This study |
| PPV20 | 3937 derivative acsA-37cbsE-1 | 16 |
| Plasmids | ||
| pCPP2228 | pCPP19 cosmid carrying the outF gene | 32 |
| p-FntA | pUC18 derivative vector carrying the ftnA gene of E. chrysanthemi | 7 |
| pBAD24 | Arabinose regulation | 21 |
| pBADI | pBAD24 derivative | 45 |
| pBADIK | pBADI derivative | 45 |
| pD-HisAcsD | pBADI derivative vector producing the N-terminally His-tagged version of AcsD | This study |
| pK-Lc | pBADIK derivative vector producing the vesicular stomatitis virus-tagged version of OutLC | 45 |
| pSH18-34 | Carrying the lexAop-lacZ reporter gene | 19 |
| pJG4-5 | Cloning vector to produce a hybrid protein containing the B42 motif | 19 |
| pB-AcsA | pJG4-5 expressing B42-AcsA hybrid protein | This study |
| pB-AcsB | pJG4-5 expressing B42-AcsB hybrid protein | This study |
| pB-AcsC | pJG4-5 expressing B42-AcsC hybrid protein | This study |
| pB-AcsD | pJG4-5 expressing B42-AcsD hybrid protein | This study |
| pB-AcsE | pJG4-5 expressing B42-AcsE hybrid protein | This study |
| pEG202 | Cloning vector to produce a hybrid protein containing LexA | 19 |
| pA-OutE | pEG202 expressing LexA-OutE hybrid protein | 45 |
| pA-OutFN | pEG202 expressing LexA-OutFN hybrid protein | 44 |
| pA-OutFC | pEG202 expressing LexA-OutFC hybrid protein | 44 |
| pA-OutLC | pEG202 expressing LexA-OutLC hybrid protein | 45 |