TABLE 2.
Achromobactin production in the E. chrysanthemi outF mutant
| Tested straina | Indicator strain growth halo diam (mm)b |
|---|---|
| A350 cbsE | 20.6 ± 1.7 |
| A350 cbsEoutF | 6.8 ± 2.1 |
| A350 cbsEoutF/pCPP2228 | 18.6 ± 0.2 |
| A350 cbsEacsD | 0 ± 0 |
Strains were grown in low-iron minimal medium until late exponential phase. Achromobactin was assayed in the culture supernatant by a bioassay using the 3937 acsA-37 cbsE-1 strain as an indicator.
The diameters of the zones of growth of the indicator strain were measured after 24 h. The data are means of three independent experiments ± SEM.