TABLE 1.

Bacterial strains used

Strain	Genotype and/or other characteristics	Source or reference
B. subtilis strains
SMY	Wild type	P. Schaeffer
LS1002	ΔamyE::Φ(citBp21-lacZ cat) ccpC::spc ccpA::Tn917(ble)	23
SG82	trpC2 lacA::tet	5
HKB5	ccpA::Tn917(ble)	SMY × LS1002 DNA
BB1888	lacA::tet	SMY × SG82 DNA
BMM1	ΔamyE::Φ(lmo1569-lmo1568-citZ-lacZ) ΔccpC::spc	BMM6 × LS1002 DNA
BMM3	ΔamyE::Φ(lmo1568-citZ-lacZ) ΔccpC::spc	BMM7 × LS1002 DNA
BMM4	ΔamyE::Φ(citZ-lacZ) ΔccpC::spc	BMM8 × LS1002 DNA
BMM6	ΔamyE::Φ(lmo1569-lmo1568-citZ-lacZ)	BB1888 × pEMM10
BMM7	ΔamyE::Φ(lmo1568-citZ-lacZ)	BB1888 × pEMM11
BMM8	ΔamyE::Φ(citZ-lacZ)	BB1888 × pEMM13
BMM12	ΔamyE::Φ(lmo1569-lmo1568-citZ-lacZ) ΔccpA::ble	BMM6 × HKB5 DNA
BMM13	ΔamyE::Φ(lmo1568-citZ-lacZ) ΔccpA::ble	BMM7 × HKB5 DNA
L. monocytogenes strains
EGD-e	Animal isolate, genome sequencing strain	14
HKB214	Δint::Φ(citB-lacZ neo)	24
LMM16	Δint::Φ(lmo1568-lacZ neo), contains 143 bp upstream of lmo1568 fused to the lacZ gene	This study
LMM17	Δint::Φ(lmo1569-lacZ neo), contains 155 bp upstream of lmo1569 and 36 bp of lmo1569 coding region fused to lacZ	This study
LMM18	Δint::Φ(lmo1568-citZ-lacZ neo), contains 203 bp upstream of lmo1568, the entire lmo1568 coding sequence, and the region between lmo1568 and citZ fused to lacZ	This study
LMM19	Δint::Φ(citZ-lacZ neo), contains 484 bp upstream of citZ fused to lacZ	This study
LMM21	Δint::Φ(lmo1568-lacZ neo) ΔccpC::spc	LMM16 × pHK95
LMM22	Δint::Φ(lmo1569-lacZ neo) ΔccpC::spc	LMM17 × pHK95
LMM23	Δint::Φ(lmo1568-citZ-lacZ neo) ΔccpC::spc	LMM18 × pHK95
LMM24	Δint::Φ(citZ-lacZ neo) ΔccpC::spc	LMM19 × pHK95
LMM33	Δint::Φ(citB-lacZ neo) ΔcitZ	HKB214 × pEMM47
SP121	Δint::Φ(lmo1568-citZ-lacZ neo) ΔccpA::tet	LMM18 × pSP65
SP122	Δint::Φ(citZ-lacZ neo) ΔccpA::tet	LMM19 × pSP65
SP124	Δint::Φ(citZ-lacZ neo) ΔccpC::spc ΔccpA::tet	LMM24 × pSP65