TABLE 2.
Oligonucleotides used in this work
| Oligonucleotide | Sequence (5′ to 3′)a | Restriction site(s) |
|---|---|---|
| OBB102 | CGGAAAGTATGTTGTTTTTACTTC | |
| OMM014 | GACTGAGAATTCGTATATAACGGCAAAACAGC | EcoRI |
| OMM015 | ACGTACGAATTCGGAAATCAAAA GAAACATACGC | EcoRI |
| OMM016 | TCGTCGGAATTCATGTTTACGGAAAGTATGTTG | EcoRI |
| OMM017 | CATGCAAAGCTTGGTACCTTCATTTCCTCCTTTTATCC | HindIII, KpnI |
| OMM041 | CCATTACTTTGCCGTCGAC | |
| OMM042 | GCTACAG CAATGATAAGCG | |
| OMM046 | CCCCAATTTATCCCTTTTGC | |
| OMM047 | GGGGCCAAAAGCCGATTAC | |
| OMM048 | ACTGCAGAATTCCAAGGTGTTGTATATAACGGC | EcoRI |
| OMM049 | TGACTGGGTACCGCCATATAAAGCCCAATAAAG | KpnI |
| OMM050 | GTACGAGAATTCCTATGGGAAAAACTTTCTATAGAGG | EcoRI |
| OMM051 | ACTGCAGGTACCGAAGTAAAAACAACATACTTTCCG | KpnI |
| OMM064 | CGACAGCTACAGCAATGATAAGCG | |
| OMM070 | AAGGCGATTAAGTTGG GTAAC | |
| OMM071 | GCGCAACTGTTGGGAAG | |
| OMM074 | GGTGTTATAACAACTATCCTTGGACTGCTC | |
| OMM085 | GAGAGTTTGATCCTGGCTC | |
| OMM086 | CCTACCAACTAGCTAATGCAC | |
| OMM087 | GTTGCTGAAACGTCCATTAGC | |
| OMM088 | CTGTATCAAATACACCGAGCTTGG | |
| OMM092 | CAACGGCCATATAGCCTACAC | |
| OMM097 | GACGGAACTGGTCCAGACATC | |
| OMM098 | GACCATATCTGTGTCTTCTGGACG | |
| OMM099 | ACTGACGGATCCAATTCGCTTATCATTGCTGTAGCTGTC | BamHI |
| OMM100 | GGCCCGATTTAAGCACACTTATTCAAGCCCTATTCTC | |
| OMM101 | GCTGAGTCTGGCTTTCGGAGAAAATCATTCAGTTTGC | |
| OMM102 | GTCACTCCATGGTGGTGAAGGAACACCTTTGAAATAACG | NcoI |
| OMM140 | ATTTATGTTGGACCTGAAAATAG | |
| OSP157 | AGCGAAAGATCTAAAACTTATTCGTGGTGG | BglII |
| OSP158 | GTAAGTCTTCTCGCAATATCTGGATCCCCACGAGCAAGTTCAGC | BamHI |
| OSP159 | CCAGATATTGCGAGAAGACTTAGTCGACATGACAAGCAACAATACG | SalI |
| OSP160 | AAATTCCCATGGGTGATTAAAGTCCATCGC | NcoI |
| OSP172 | TCCAGACCCGGGAGTTGCATTATCG | SmaI |
| OSP174 | TGCGTACCCGGGCTTCATCTGTTTGCTGG | SmaI |
a
Some of the oligonucleotides contained recognition sites for restriction enzymes, which are indicated by underlining.