TABLE 3.
Effects of ccpC or ccpA mutations on expression of L. monocytogenes citZ in B. subtilis
| Fusion | β-Galactosidase activity (Miller units)a
|
||
|---|---|---|---|
| Wild type | ccpC null mutant | ccpA null mutant | |
| Long [(lmo1569-lmo1568-citZ)-lacZ] | 11 ± 0.8 | 62 ± 0.8 | 10 ± 2.5 |
| Medium [(lmo1568-citZ)-lacZ] | 0.9 ± 0.2 | 38 ± 0.2 | 0.6 ± 0.03 |
| Short (citZ-lacZ) | 0.7 ± 0.1 | 0.38 ± 0.01 | ND |
a
β-Galactosidase activity was determined for B. subtilis wild-type, ccpC null mutant, and ccpA null mutant strains carrying fusions to lacZ of segments of the citZ locus (Fig. 1). The organisms were grown at 37°C in TSS medium supplemented with 0.5% glucose and 1.6 mg glutamine per ml, and cells were harvested at several times during exponential growth phase and used for analysis. No growth phase-related regulation was seen. The β-galactosidase activities shown are the averages ± standard deviations of three independent experiments, in each of which samples were removed at multiple time points. ND, not determined.