TABLE 4.
Effects of ccpC, ccpA, and ccpA ccpC mutations on citZ expression in L. monocytogenes
| Fusion | β-Galactosidase activity (Miller units)
|
|||
|---|---|---|---|---|
| Wild type | ccpC mutant | ccpA mutant | ccpC ccpA mutant | |
| lmo1569-lacZ (A) | 24 ± 2.0 | 27 ± 2.4 | ND | ND |
| lmo1568-lacZ (B) | 5.0 ± 1.5 | 24 ± 3.2 | ND | ND |
| (lmo1568-citZ)-lacZ (C) | 3.2 ± 1.5 | 24 ± 5 | 3.9 ± 0.4 | ND |
| citZ-lacZ (D) | 0.5 ± 0.1 | 0.3 ± 0.05 | 0.6 ± 0.2 | 0.7 ± 0.4 |
a β-Galactosidase activity was determined for wild-type, ccpC null mutant, ccpA null mutant, and ccpC ccpA mutant strains of L. monocytogenes carrying various lacZ fusions. The organisms were grown at 37°C in BHI medium. The results shown are the averages ± standard deviations of three independent experiments. ND, not determined.