TABLE 2.

Oligonucleotides used for gene-specific real-time PCR in A. phagocytophilum

Target gene	Direction	Sequence (5′-3′)	Amplicon size (bp)
pleC	Forward	TGATAAGGGCGACATTGGTTGTGC	89
	Reverse	AGCTGCTTTGCTTAGCGTTCTGTC
pleD	Forward	ACATGCGTACAAACCCTGCCATTG	109
	Reverse	AAATCATCTGCACCAGCACTCAGC