TABLE 2.

Reproducibility of method B for NoV detection for six naturally contaminated samples, each extracted three times

Sample	Genogroup I NoV				Genogroup II NoV
	rRT-PCR efficiency (%)a	Concnb (RNA copies/g DT)			rRT-PCR efficiency (%)a	Concnb (RNA copies/g DT)
		Assay 1	Assay 2	Assay 3		Assay 1	Assay 2	Assay 3
1	97.0 ± 1.7	330	+DLc		100.7 ± 0.6	16	43	38
2	95.6 ± 2.5				100.9 ± 2.6			+DL
3	94.8 ± 4.3				98.2 ± 5.2
4	96.7 ± 1.9			250	102.4 ± 0.3	110	+DL	88
5	97.6 ± 1.7	840			99.7 ± 3.9	190	58
6	94.8 ± 5.0	910			100.1 ± 1.8	110	+DL	53

^arRT-PCR efficiency was calculated based on coamplification of genogroup I and II RNA external controls with pure and 10-fold-diluted nucleic acid extract.

^bThe genogroup I or II NoV concentration was calculated based on C_T values obtained for pure and 10-fold-diluted nucleic acid extract and the corresponding standard curve.

^c+DL, positive sample, but the level was too close to the limit of detection for quantification.