TABLE 2.
Horizontal cotransmission of the S phenotype and the putative viral markera
Donor
|
One generation after transfer (emerging F1)
|
Later after transfer
|
||||||
---|---|---|---|---|---|---|---|---|
Lineb | Phenotype (n)c | No. of positive PCR tests/no. of individuals tested | No. of females tested | PCR results | n | No. of generations | Phenotypec | No. of positive PCR tests/no. of individuals tested |
G9 | 3.3 ± 0.75 (6) | 3/3d | 3 | + | 1 | 15 | 3.60 ± 0.33 | 4/4e |
− | 2 | |||||||
G10 | 3.9 ± 0.52 (6) | 3/3d | 11 | + | 6 | 15 | 3.35 ± 0.22 | 5/5e |
− | 5 | |||||||
G11 | 5.3 ± 0.81 (6) | 3/3d | 3 | + | 0 | |||
− | 3 | |||||||
G12 | 5.7 ± 1.17 (6) | 3/3d | 2 | + | 2 | 15 | 3.31 ± 0.30 | 5/5e |
− | 0 | |||||||
G13 | 7.1 ± 1.25 (6) | 3/3d | 5 | + | 0 | |||
− | 5 | |||||||
G14 | 7.2 ± 0.61 (6) | 3/3d | 14 | + | 0 | |||
− | 14 | |||||||
G15 | 8 ± 1.62 (6) | 3/3d | 4 | + | 2 | 15 | 2.68 ± 0.34 | 5/5e |
− | 2 | |||||||
G16 | 9.3 ± 1.93 (6) | 3/3d | 6 | + | 3 | 15 | 3.55 ± 0.12 | 5/5e |
− | 3 | |||||||
G17 | 11.9 ± 1.80 (6) | 3/3d | 6 | + | 3 | 15 | 3.57 ± 0.07 | 5/5e |
− | 3 | |||||||
G18 | 5.88 ± 1.51 (5) | 18/18e | 21 | + | 3 | 4 | 2.51 ± 0.11 | 9/9e |
− | 18 | |||||||
Ep1 | 6.48 ± 0.89 (5) | 4/4e | 5 | + | 1 | 4 | 2.19 ± 0.07 | 9/9e |
− | 4 | |||||||
Ep2 | 8.18 ± 1.05 (5) | 11/11e | 10 | + | 0 | |||
− | 10 | |||||||
P2 | 2.89 ± 0.72 (6) | 18/18e | 18 | + | 7 | 4 | 2.29 ± 0.10 | 9/9e |
− | 11 |
The recipient lines were founded by females belonging to the NSref line. In each case before transfer the recipient had the NS phenotype (1.1 ± 0.01 parasitic larvae per parasitized host), and a PCR was negative.
The donor lines were S phenotype lines from different geographical areas. G9 to G18, Gotheron lines; Ep1 and Ep2, Epinouze lines; P2, Palma de Mallorca line.
The phenotype is expressed as the number of parasitic larvae per parasitized host (mean ± standard error).
Uniplex PCR.
Multiplex PCR.