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Total AK specific activity in crude extracts of shake flask cultures of B. methanolicus MGA3 and recombinant strains overexpressing dapG, lysC, and yclM. For each strain, the AK activity is given relative to the activity of control strain MGA3(pHP13) (which was defined as 1), which had a measured total AK specific activity of 0.05 U/mg protein.
