TABLE 2.
PCR primers used in this study
| Primer | Sequence (5′-3′)a |
|---|---|
| mlpA-PPS-1F | TCTACCTTCGTTGAGGAAGA |
| asd-PPS-1R | CACTCCTGAACGGTTAATCC |
| asd-PPS-1F | TGAGCAGACAAGAGCGATTA |
| ymfA-PPS-1R | ATAGATCGCTCCGATATGGT |
| yclM-PPS-1F | CCTGTGATCGGAATTGCAAGTGATAAAGGATTCTG |
| yclM-PPS-1R | ATCTTCGTTCCAGGAGCCGATGGATTATTGGTGTT |
| mdh-CDS-F1 | TCGACATGTGACAACAAACTTTTTC |
| pTB1.9-R1 | ACGCATACCATTTTGAACGATGACC |
| mdh-prom-F1 | GCCGGATCCTGCAGTTCATTAAAGAGCAGC |
| mdh-prom-R1 | CGCGACATGTACTACCTCCTATTTATG |
| mp-mdh-P2-F1 | CGCGGCATGCGTTTCAATGAAGATCC |
| mp-mdh-P2-R1 | TTAAGCATGCAAAAGGCCAGGAACCG |
| mp-mdh-P2-F2 | TTTTGGTACCCGCCATAGGTCTAGAG |
| mp-mdh-P2-R2 | GGGCGGTACCTTATTCTTTAGTCTATC |
| lysC-CDS-F1 | CCGAACATGTGGGATTAATTGTCC |
| lysC-CDS-R1 | TTCCGGTACCCAGCAAATTGAACAGC |
| dapG-CDS-F1 | GCGCACATGTGAAAATTATCGTTCAAAAATTCGG |
| dapG-CDS-R1 | GCTAGGTACCGCTCCTCCTCATTCTATC |
| yclM-CDS-F1 | GCGCACATGTGAAAGTAGCGAAGTTTGGAGGTTCTTC |
| yclM-CDS-R1 | GCTAGGTACCAGTGTTTCACACCCAAATTCG |
| dapG-fwd1 | AAAGGTACCCATATGAAAATTATCGTTCAAAAATTCGG |
| dapG-rev1 | AACTCGAGTTCTATCCGTTCAAACTCCAG |
| lysC-fwd1 | CTACATGTCCATATGGGATTAATTTGTCCAAAAGTTTGG |
| lysC-rev1 | CGTGAAGTCGACGCGTTCCGATTTAACAGCGGATC |
| yclM-fwd1 | ATCGCTATACATATGAAAGTAGCGAAGTTTGGAGGTTC |
| yclM-rev1 | AACTCGAGAACATTTACAGGAACTGGAGC |
a
The underlined nucleotides are restriction sites used for simplified cloning of PCR products.