TABLE 3.
Initial specific growth rates, maximum dry weights of cells, and l-lysine production for B. methanolicus wild-type strain MGA3 and recombinant strains overexpressing dapG, lysC, and yclM
| Strain | Specific growth rate (h−1) | Dry wt of cells (g/liter)a | l-Lysine production (g/liter)a | l-Lysine concn in growth medium (g/liter)b |
|---|---|---|---|---|
| MGA3 | 0.49 | 58 | 0.18 | 0.12 |
| MGA3(pHP13) | 0.49 | 56 | 0.18 | 0.12 |
| MGA3(pHP13mp-dapG) | 0.50 | 62 | 0.38 | 0.23 |
| MGA3(pHP13mp-lysC) | 0.46 | 61 | 1.8 | 1.1 |
| MGA3(pHP13mp-yclM) | 0.50 | 54 | 11 | 7.0 |
a
The biomass and l-lysine production values were corrected for dilution caused by feeding throughout the fermentation in order to compare results from different bioreactor trials (see Materials and Methods).
b
l-Lysine concentration in growth medium (no volume correction).