TABLE 3.
Concentrations of NH3 (aq) in solution calculated from maximum pH, temperature, and total ammonia, together with time 99% inactivation (t99) of viable A. suum eggs
| Material | Treatment | Maximum pH | 34°C
|
24°C
|
14°C
|
4°C
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NH3 (aq)
|
t99 (days)a
|
NH3 (aq)
|
t99 (days)
|
NH3 (aq)
|
t99 (days)
|
NH3 (aq)
|
t99 (days)
|
|||||||||||
| mM (mean ± SD) | % | Mean | 95% | mM (mean ± SD) | % | Mean | 95% | mM (mean ± SD) | % | Mean | 95% | mM (mean ± SD) | % | Mean | 95% | |||
| Feces | U2 | 9.0 | 440 ± 40 | 51 | 3.8 | 6.4 | 230 ± 20 | 35 | 28 | 33 | ||||||||
| U1 | 8.9 | 250 ± 4 | 46 | 4.1 | 5.9 | 130 ± 9 | 30 | 47c | 59 | |||||||||
| AU1 | 12.8 vs 10 | 72 ± 6 | 100 | 3.8 | 6.4 | 220 ± 26 | 84 | 13 | 18 | |||||||||
| A | 12.8 vs 10.5 | 71 ± 6 | 100 | 3.7 | 4.1 | 57 ± 6 | 94 | 35c | 50 | |||||||||
| U0 | 8.3 | 43 ± 2 | 17 | 21 (8) | 27 | 20 ± 0.4 | 10 | 74c | 114 | |||||||||
| Urine | 1:0 | 9.0, 9.1b | 236 ± 8 | 54 | 3.4 (≥1) | 4.0 | 141 ± 11 | 34 | 48 | 62 | 94 ± 3 | 22 | 240c | 380 | 57 ± 6 | 13 | 480c | 770 |
| 1:1 | 8.9, 8.9 | 100 ± 8 | 46 | 6.3 (≥2) | 7.3 | 66 ± 2 | 30 | 56 (17)c | 70 | 39 ± 10 | 17 | 1,060c | 1,250 | 16 ± 0.3 | 8 | 840c | 1,050 | |
| 1:3 | 8.7, 9.1 | 40 ± 31 | 44 | 8.5 (≥2) | 14 | 18 ± 11 | 22 | —d | 20 ± 6 | 20 | —d | 14 ± 1 | 14 | —d | ||||
a
Predicted by linear regression of data sets with significant inactivation and given as mean and upper 95th percentile. The lag phase, when present, is given in parentheses with the predicted time (days) for t99.
b
The first value is for 34 and 24°C, and the second is for 14 and 4°C.
c
t99 not confirmed with zero viable larvae.
d
Regression coefficient not significantly (P > 0.05) different from zero.