TABLE 2.

Production of glucaric acid in BL21 Star (DE3)(pRSFD-IN-MI, pTrc-udh) after 3 days of culture^a

Conditionb	OD600c	Concn (g/liter) of:				Yield (%)d
		Glucose	myo-Inositol	Glucuronic acid	Glucaric acid
A	5.10 ± 0.27	5.69 ± 0.85	0.10 ± 0.02	NDe	0.72 ± 0.09	17.4 ± 5.1
B	6.13 ± 0.31	1.43 ± 0.81	0.18 ± 0.05	ND	1.13 ± 0.17	13.1 ± 1.0
C	5.80 ± 0.39	2.47 ± 1.00	0.23 ± 0.07	ND	0.82 ± 0.06	11.0 ± 2.4

^aCultures were grown at 30°C in LB medium supplemented with 10 g/liter glucose and induced with IPTG. Data are the averages and standard deviations of three independent experiments.

^bCondition A, 0.1 mM IPTG at 0 h; condition B, 0.05 mM IPTG at 0 h; condition C, 0.05 mM IPTG at 0 h and 0.1 mM IPTG at 17.5 h.

^cOD₆₀₀, optical density at 600 nm.

^dYield = 100 × glucaric acid produced/glucose consumed (mol/mol).

^eND, not detectable.