TABLE 3.
Determination of electron acceptors in the NADH oxidase and DHO:NAD+ oxidoreductase reactions of B. bifidum DHOD
| Electron acceptor | Relative activity (%)a
|
|
|---|---|---|
| NADH:acceptor oxidoreductase | DHO:acceptor oxidoreductase | |
| O2 | 100 | 14 |
| DCIP | 410 | 88 |
| Menadione | 187 | 54 |
| Q0 | 118 | 51 |
| Q10b | NDc | ND |
| NAD+ | —d | 24 |
| Cytochrome c | 144 | 33 |
| Orotate | 142.5 | — |
| Fumarate | ND | ND |
a
Relative activity (percent) was calculated relative to the activity of NADH:oxygen oxidoreductase.
b
Q10 was dissolved in 100% ethanol, and 10 μl of the solution was added to the assay solution (final volume, 1 ml).
c
ND, not detected.
d
—, cannot be used as a substrate in this assay.