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. 2008 Dec 1;75(3):687–694. doi: 10.1128/AEM.01506-08

FIG. 4.

FIG. 4.

Product formation during bioreduction of ethyl 3-keto-4,4,4-trifluorobutyrate (compound 1) by using coupled permeabilized cells with 0.005 mM NADP+ added at different time points. •, B. pumilus Phe-C3 (40 g [dry weight] of cells per liter) and E. coli BL21(pGDH1) (20 g [dry weight] of cells per liter; 61 U/g [dry weight] of cells) with 120 mM 3-ketoester 1; □, B. pumilus Phe-C3 (40 g [dry weight] of cells per liter) and E. coli BL21(pGDH1) (20 g [dry weight] of cells per liter; 61 U/g [dry weight] of cells) with 60 mM 3-ketoester 1. 2, (R)-ethyl 3-hydroxy-4,4,4-trifluorobutyrate.