FIG. 1.

Biotransformations of 2-MIB by camphor-grown bacteria. Time series incubations were carried out as described in Materials and Methods. Samples were taken at the beginning and at time intervals, extracted with methylene chloride, and analyzed by GC-MS. (A) R. ruber T1. The optical density at 600 nm (OD₆₀₀) of strain T1 at the beginning was 2.5. In an identical experiment using a similar density of strain T1 grown in the absence of camphor, no 2-MIB had been transformed after 3.5 h. (B) P. putida G1. The OD₆₀₀ of strain G1 at the beginning was 3.7. In an identical experiment using a similar density of strain G1 grown in the absence of camphor, less than 20% of 2-MIB had been consumed after 5 h. (C) R. wratislaviensis DLC-cam. The OD₆₀₀ of strain DLC-cam at the beginning was 1.3. In an identical experiment using a similar density of strain DLC-cam grown in the absence of camphor, no 2-MIB had been transformed after 6 h.