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Detection of nirK-type denitrifiers in activated sludge by using RING-FISH with nirK polynucleotide probes. (A and C) Halo-shaped fluorescence signals of Cy3-labeled denitrifiers. (B) Floc in phase-contrast micrograph. (D) Bacterial cells in sludge stained by oligonucleotide probes EUB338I to EUB338III. Bars = 10 μm.
