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. 1984 Aug;45(2):356–359. doi: 10.1128/iai.45.2.356-359.1984

Effect of fluoride on growth and acid production by Streptococcus mutans in dental plaque.

J S van der Hoeven, H C Franken
PMCID: PMC263229  PMID: 6746094

Abstract

The aim of this study was to measure the effect of fluoride on the production of organic acids by Streptococcus mutans in dental plaque. The effect was studied in a simplified model of dental plaque with gnotobiotic rats monoinfected with S. mutans Ny341. Adaptation of S. mutans to fluoride was induced by feeding one group of the rats on fluoride-containing diet and drinking water. No difference was found in the accumulation of S. mutans on the teeth between the fluoride-adapted and the control groups. However, there was a significant difference in the amount of lactic acid in metabolically resting plaque between the groups, lactic acid being lower in the fluoride-adapted plaque. At 5 min after a rinse containing 10% sucrose, a high level of lactic acid was found in plaque from animals not exposed to fluoride. Rinses containing 4 or 20 mM fluoride before the sucrose rinse significantly inhibited the lactic acid production in the control group. In the plaque from rats on fluoridated diet and drinking water the sucrose-induced production of lactic acid was not inhibited by a 4 mM fluoride rinse. Moreover, the production of lactic acid in the fluoride-adapted plaque was prolonged. The results indicate that due to fluoride adaptation the inhibition of acid production is unlikely to be important for the caries-preventive action of fluoride.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beighton D., Colman G. A medium for the isolation and enumeration of oral actinomycetaceae from dental plaque. J Dent Res. 1976 Sep-Oct;55(5):875–878. doi: 10.1177/00220345760550052701. [DOI] [PubMed] [Google Scholar]
  2. Bowden G. H., Odlum O., Nolette N., Hamilton I. R. Microbial populations growing in the presence of fluoride at low pH isolated from dental plaque of children living in an area with fluoridated water. Infect Immun. 1982 Apr;36(1):247–254. doi: 10.1128/iai.36.1.247-254.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brown W. E., Gregory T. M., Chow L. C. Effects of fluoride on enamel solubility and cariostasis. Caries Res. 1977;11 (Suppl 1):118–141. doi: 10.1159/000260298. [DOI] [PubMed] [Google Scholar]
  4. Bunick F. J., Kashket S. Enolases from fluoride-sensitive and fluoride-resistant streptococci. Infect Immun. 1981 Dec;34(3):856–863. doi: 10.1128/iai.34.3.856-863.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. De Stoppelaar J. D., Van Houte J., Backer Dirks O. The relationship between extracellular polysaccharide-producing streptococci and smooth surface caries in 13-year-old children. Caries Res. 1969;3(2):190–199. doi: 10.1159/000259582. [DOI] [PubMed] [Google Scholar]
  6. Donkersloot J. A., Robrish S. A., Krichevsky M. I. Fluorometric determination of deoxyribonucleic acid in bacteria with ethidium bromide. Appl Microbiol. 1972 Aug;24(2):179–183. doi: 10.1128/am.24.2.179-183.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Eisenberg A. D., Bender G. R., Marquis R. E. Reduction in the aciduric properties of the oral bacterium Streptococcus mutans GS-5 by fluoride. Arch Oral Biol. 1980;25(2):133–135. doi: 10.1016/0003-9969(80)90089-8. [DOI] [PubMed] [Google Scholar]
  8. Eisenberg A. D., Marquis R. E. Enhanced transmembrane proton conductance in Streptococcus mutans GS-5 due to ionophores and fluoride. Antimicrob Agents Chemother. 1981 May;19(5):807–812. doi: 10.1128/aac.19.5.807. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hamilton I. R., Bowden G. H. Response of freshly isolated strains of Streptococcus mutans and Streptococcus mitior to change in pH in the presence and absence of fluoride during growth in continuous culture. Infect Immun. 1982 Apr;36(1):255–262. doi: 10.1128/iai.36.1.255-262.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hamilton I. R. Effects of fluoride on enzymatic regulation of bacterial carbohydrate metabolism. Caries Res. 1977;11 (Suppl 1):262–291. doi: 10.1159/000260304. [DOI] [PubMed] [Google Scholar]
  11. Hamilton I. R., Ellwood D. C. Effects of fluoride on carbohydrate metabolism by washed cells of Streptococcus mutans grown at various pH values in a chemostat. Infect Immun. 1978 Feb;19(2):434–442. doi: 10.1128/iai.19.2.434-442.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jenkins G. N. The mechanism of action of fluoride in reducing caries incidence. Int Dent J. 1967 Sep;17(3):552–563. [PubMed] [Google Scholar]
  13. Kilian M., Thylstrup A., Fejerskov O. Predominant plaque flora of Tanzanian children exposed to high and low water fluoride concentrations. Caries Res. 1979;13(6):330–343. doi: 10.1159/000260423. [DOI] [PubMed] [Google Scholar]
  14. Moreno E. C., Kresak M., Zahradnik R. T. Physicochemical aspects of fluoride-apatite systems relevant to the study of dental caries. Caries Res. 1977;11 (Suppl 1):142–171. doi: 10.1159/000260299. [DOI] [PubMed] [Google Scholar]
  15. Sandham H. J., Kleinberg I. Effect of fluoride on carbon dioxide and acid formation in salivary sediment mixtures incubated with glucose. Arch Oral Biol. 1973 Feb;18(2):211–225. doi: 10.1016/0003-9969(73)90141-6. [DOI] [PubMed] [Google Scholar]
  16. Shields W. F., Mühlemann H. R. Simultaneous pH and fluoride telemetry from the oral cavity. Helv Odontol Acta. 1975 Apr;19(1):18–26. [PubMed] [Google Scholar]
  17. Streckfuss J. L., Perkins D., Horton I. M., Brown L. R., Dreizen S., Graves L. Fluoride resistance and adherence of selected strains of Streptococcus mutans to smooth surfaces after exposure to fluoride. J Dent Res. 1980 Feb;59(2):151–158. doi: 10.1177/00220345800590021501. [DOI] [PubMed] [Google Scholar]
  18. van Houte J., Aasenden R., Peebles T. C. Oral colonization of Streptococcus mutans in human subjects with low caries experience given fluoride supplements from birth. Arch Oral Biol. 1978;23(5):361–366. doi: 10.1016/0003-9969(78)90093-6. [DOI] [PubMed] [Google Scholar]
  19. van der Hoeven J. S., Franken H. C., Camp P. J., Dellebarre C. W. Analysis of bacterial fermentation products by isotachophoresis. Appl Environ Microbiol. 1978 Jan;35(1):17–23. doi: 10.1128/aem.35.1.17-23.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. van der Hoeven J. S., Franken H. C. Production of acids in rat dental plaque with or without Streptococcus mutans. Caries Res. 1982;16(5):375–383. doi: 10.1159/000260623. [DOI] [PubMed] [Google Scholar]

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