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. Author manuscript; available in PMC: 2009 Dec 5.
Published in final edited form as: J Mol Biol. 2008 Sep 16;384(1):219–227. doi: 10.1016/j.jmb.2008.09.021

Table III.

Inhibition constants of PR11 and some its most active mutants.

Peptide Ki (nM) Error
PR11 (wild type) 86.5 21.1
W12 39.5 2.3
F8W12 184 16.3
A8W12 50.7 16.8

Conditions: 5 nM human 20S proteasome, 20–100 µM Suc-LLVY-AMC substrate, 0, 75 or 100 nM PR-peptide, 25 mM Tris, pH 7.5, 0.03% SDS, 37 °C. Under these conditions, Km for the enzyme was 47.4±5 µM and Vmax was 0.55±0.03 µmol/h/mg.