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. Author manuscript; available in PMC: 2009 Jan 28.
Published in final edited form as: J Neurovirol. 2007 Oct;13(5):433–445. doi: 10.1080/13550280701460565

Figure 7.

Figure 7

Administration of neutralizing antibody to VSV prevents splenic IFN-β mRNA production. Anti-VSV polyclonal Ab was administered i.p. either 2 h prior to infection (top panels) or 6 h after i.n. application of 1 × 104 PFU virus (bottom panels) to groups of three BALB/c mice. Controls included i.n. mock-infection with PBS and i.p. injection of normal goat serum. mRNA was harvested at 24 h. p.i. from brains or spleens and RT-PCR was performed for amplification of VSV M protein, IFN-β or GAPDH message. Where viral gene expression was observed, IFN-β mRNA was produced. Anti-VSV Ab neutralized virus and prevented splenic mRNA production if present before infection.