Table 2.
Specification of transcripts showing similarity to enzymes involved in starch and lipid reserve mobilization.a
| Reaction | Enzyme | GenBank accession number of EST showing similarity to the enzyme | Cluster | Maximal absolute expression under standard conditions | Relative expression under standard conditions |
| Starch degradation | |||||
| 1 | Alpha-amylase (EC 3.2.1.1) | FG345587 | k2.2 | 4240 | 148.2 |
| 1 | Alpha-amylase (EC 3.2.1.1) | FG344655 | k2.1 | 1820 | 43.1 |
| 2 | Beta-amylase (EC 3.2.1.2) | FG345593 | - | 490 | 5.1 |
| 2 | Beta-amylase (EC 3.2.1.2) | FG344086 | - | 205 | 0.3 |
| 3 | Alpha-glucosidase (EC 3.2.1.20) | FG344078 | - | 5 | 1.0 |
| Glycolysis | |||||
| 4 | Phosphoglucomutase (EC 5.4.2.2), cytosolic | BQ586122 | - | 70 | 1.0 |
| 5 | UDP-glucose pyrophosphorylase (EC 2.7.7.9) | FG343121 | - | 110 | 3.2 |
| 6 | Phosphofructokinase (PFK) (EC 2.7.1.11) | FG343255 | k1.1 | 40 | 0.1 |
| 7 | Pyrophosphate-fructose 6-phosphate 1-phosphotransferase (PFP) (EC 2.7.1.90) | FG345703 | k2.2 | 180 | 2.7 |
| 8 | Fructose-bisphosphate aldolase (EC 4.1.2.1), cytosolic | FG343104 | - | 510 | 2.7 |
| 8 | Fructose-bisphosphate aldolase (EC 4.1.2.1), plastidic | FG345732 | k2.2 | 180 | 10.4 |
| 9 | Triosephosphate isomerase (TPI) (EC 5.3.1.1), cytosolic | FG344285 | k2.2 | 400 | 7.9 |
| 10 | Glyceraldehyde-3-P dehydrogenase (GAPDH) (EC 1.2.1.12), cytosolic | FG345028 | k2.2 | 1980 | 2.3 |
| 11 | Phosphoglycerate kinase (PGK) (EC2.7.2.3), cytosolic | FG343147 | k2.2 | 575 | 3.8 |
| 12 | Phosphoglycerate mutase (PGAM) (EC 5.4.2.1), cytosolic | BQ583729 | - | 35 | 2.4 |
| 13 | Enolase (ENO) (EC 4.2.1.11), cytosolic | FG345118 | k2.1 | 440 | 4.1 |
| 14 | Pyruvate kinase (PK) (EC 2.7.1.40), cytosolic | FG344735 | k2.1 | 210 | 2.1 |
| Pyruvate decarboxylation and fermentation | |||||
| 15 | Pyruvate dehydrogenase E1 alpha subunit (EC 1.2.4.1) | FG345415 | k2.1 | 80 | 4.9 |
| 16 | Alcohol dehydrogenase (EC 1.1.1.1) | FG345166 | k2.2 | 30 | 3.0 |
| 16 | Alcohol dehydrogenase (EC 1.1.1.1) | FG343474 | - | 460 | 0.5 |
| Fatty acid beta-oxidation | |||||
| 17 | Acyl-CoA synthetase (EC 6.2.1.3) | FG344059 | - | 165 | 3.5 |
| 18 | Acyl-CoA oxidase ACX3 (EC 1.3.3.6) | FG345045 | - | 120 | 1.5 |
| 19a | Enoyl-CoA hydratase (EC 4.2.1.17) | FG345194 | k2.1 | 40 | 5.6 |
| 19b | Multifunctional protein (EC 4.2.1.17, EC 1.1.1.35) | FG344653 | - | 625 | 1.3 |
| 20 | Thiolase (EC 2.3.1.9) | FG344716 | k2.1 | 295 | 10.4 |
| Glyoxylate cycle | |||||
| 21 | ATP citrate synthase (EC 2.3.3.8) | FG345121 | - | 80 | 6.6 |
| 22 | Aconitate hydratase (EC 4.2.1.3) | FG345009 | k2.2 | 480 | 4.6 |
| 23 | Isocitrate lyase, glyoxysomal (EC 4.1.3.1) | FG345539 | k2.1 | 1970 | 9.4 |
| 24 | Malate synthase, glyoxysomal (EC 2.3.3.9) | FG344826 | - | 690 | 2.5 |
| 25 | Malate dehydrogenase (EC 1.1.1.37) | FG345685 | k2.2 | 575 | 2.6 |
| Gluconeogenesis | |||||
| 26 | Phosphoenolpyruvate carboxykinase (EC 4.1.1.49) | FG343814 | k2.2 | 475 | 15.3 |
| Protein catabolism | |||||
| - | Cysteine proteinase (EC 3.4.22.-) | FG344171 | k2.1 | 1880 | 25.5 |
| - | Cysteine-type endopeptidase (EC 3.4.22.1) | FG345664 | k2.1 | 1700 | 85.0 |
| - | Cysteine proteinase (EC 3.4.22.-) | FG345425 | k2.2 | 505 | 50.5 |
a Enzymes and the corresponding transcripts are arranged according the metabolic scheme shown in Figure 5. Relative expression indicates maximal fold of increment during germination.