Table 2.

Serum levels and adipose tissue messenger RNA expression of adiponectin, resistin and monocyte chemoattractant protein-1 (MCP-1) of rats fed a standard chow (n= 6) or a high fat diet (n= 6)

	Standard chow (n= 6)	High-fat diet (n= 6)	P
Fat tissue adiponectin (adiponectin mRNA /β-actin mRNA; mean value ± SEM)	0·95 ± 0·1	0·88 ± 0·1	0·7
Fat tissue resistin (resistin mRNA/β-actin mRNA; mean value ± SEM)	1·75 ± 0·4	1·2 ± 0·2	0·3
Fat tissue MCP-1 (MCP-1 mRNA/β-actin mRNA; mean value ± SEM)	1·19 ± 0·1	1·77 ± 0·39	0·2
Serum adiponectin (ng/ml) (mean value ± SEM)	5833 ± 506	6450 ± 413	0·4
Serum MCP-1 (pg/ml) (mean value ± SEM)	24·2 ± 4·8	22·4 ± 4·1	0·7
Serum resistin (ng/ml) (mean value ± SEM)	28·7 ± 2·5	30·2 ± 2·9	0·7

Messenger RNA (mRNA) was prepared from abdominal adipose tissue and analysed for β-actin and adipokine mRNA expression using quantitative LightCycler real-time reverse transcription–polymerase chain reaction. The adipokine/β-actin ratio is given as mean value ± standard error of the mean (SEM). The serum levels of adiponectin, resistin and MCP-1 were measured by enzyme-linked immunosorbent assay.