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. 2009 Feb;126(2):256–267. doi: 10.1111/j.1365-2567.2008.02894.x

Figure 7.

Figure 7

Effects of purified regulatory T cells (Tregs) on αβ T-cell proliferation. (a) Peripheral blood mononuclear cells (PBMC) from cytomegalovirus (CMV)-seropositive healthy donors were re-stimulated with CMV pp65-loaded mature dendritic cells (DCs) alone or co-cultured with autologous purified Tregs (ratio PBMC:Treg = 1 : 4) or supernatant (SN) from overnight-cultivated Tregs (ratio PBMC:Treg supernatant = 1 : 1). After 1 week, proliferation of CMV pp65-specific CD8+αβ T cells was determined by flow cytometry. Each bar represents mean values ± standard deviation of triplicate cultures from one representative donor. (b) In a mixed lymphocyte reaction (MLR), carboxyfluorescein succinimidyl ester (CFSE)-labelled PBMC were incubated with allogeneic immature dendritic cells (ratio 1 : 10) alone or together with supernatant from overnight-cultivated autologous purified Tregs (ratio PBMC:Treg supernatant = 1 : 1). On day 6, cells were analysed by flow cytometry. αβ T-cell receptor (TCR)/CD3 double-positive T cells were gated, and loss of CFSE represents proliferation of gated cells. One representative donor of three is shown.